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The possible protein protein interactions between AtMAP65 l, AtMAP65 6 and AtMAP65 5 were assessed with yeast two hybrid assays and it was shown that AtMAP65 6 interacts with itself An antibody was raised against AtMAP65 6 and used for investigating the AtMAP65 6 localisation to MT structures through the cell cycle. AtMAP65 6 does not bind to cortical MTs, however localises to preprophase band, anaphase spindle and phragmoplast. The anti AtMAP65 6 antibody recognises three isoforms on a western blot of a 2D SDS PAGE gel of total protein extract of Arabidopsis tissue culture cells..
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